- 1). Locate the urine stain on the mattress by looking for fabric discoloration or sniffing around each portion of the mattress.
- 2). Pour white distilled vinegar on the stain until it is thoroughly saturated, and allow it to set for approximately five minutes. The vinegar will soak through the mattress and kill bacteria as it absorbs odor.
- 3). Blot the vinegar out of the mattress with paper towels. Apply pressure to the towels by leaning into them or by setting heavy objects on them, and repeat this process with dry paper towels until you have absorbed most of the vinegar.
- 4). Pour baking soda over the area, and leave it there overnight. Baking soda will absorb some of the remaining moisture while neutralizing odor.
- 5). Vacuum the baking soda from the mattress.
- 6). Scrub the mattress with upholstery cleaner, if desired, according to the product's directions.
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