- 1). Pour the distilled water into a large stock pot and bring the water to a rolling boil.
- 2). Remove the lid from the empty glass bottle and, using tongs, submerge the bottle and lid into the boiling water. Boil the bottle and lid for 15 minutes.
- 3). Wash your hands with antibacterial soap.
- 4). Use the tongs to remove the bottle and lid from the boiling water, making sure the bottle is as full of the boiled distilled water as possible. Try not to touch the inside of the bottle or its lip with the tongs. Use the tongs to put the lid on the bottle and close it tightly.
- 5). Prepare the hydrochloric acid sterilization solution by adding 0.25 oz. of concentrated HCl to about 3.5 oz. of boiled distilled water in a clean container. Dilute the solution with boiled distilled water to a final volume of 8.5 oz.
- 6). Immerse the pH electrode into the sterilization solution for 1 minute. Remove the electrode and rinse it with boiled distilled water. At this point, the electrode is sterilized and should not be touched or brought into contact with nonsterile surfaces before it is used to take the pH measurement.
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