- 1). Salmonella is identified through culture of the faeces sample. This often happens when the faeces sample accompanies the symptoms of fever and food poisoning. In some cases, Salmonella typhi is identified from the culture of a blood sample.
- 2). Try first the classic approaches. There are a number of traditional techniques for identifying Salmonella, the most common being serological testing and biochemical testing. Serological testing is an analysis that examines the physical and chemical properties of a blood sample. It helps in detecting the serum antibodies associated with Salmonella typhi. Biochemical tests include the popular Agar Media Test followed by other tests, including the Triple Sugar Iron Agar or TSI test and the indole test.
- 3). Use rapid methods if you lack time. A combination of immunomagnetic separation (IMS) and polymerase chain reaction (PCR) are successfully used to identify Salmonella Typhi. These methods are widely used in commercial setups and reduces the detection time to lesser than 48 hours. The tests in rapid methods are based on enzyme immunoassay (EIA) and latex agglutination and prove to be very effective.
- 4). Try molecular methods if you have experience with them. The two most widely used molecular methods include DNA hybridization and PCR assays. These tests are used for distinguishing between different bacterial strains.
Identifying Salmonella Typhi
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